Protein Carriers

An important factor when designing synthetic peptides for use in antibody production is the method used to couple the peptide sequence to a protein carrier.  Most coupling methods rely on the presence of free amino groups, found in lysine side chains or on amino-terminal residue, sulfhydryl groups found on cysteine, phenolic groups found on tyrosine, carboxylic acid groups found on aspartic acid, glutamic acid and the carboxy terminal residue.

The easiest strategy to manipulate the type of coupling is to add an extra amino acid on either the amino or carboxy terminus to allow simple one-site coupling to the carrier.

Many different carrier proteins can be used for coupling with synthetic peptides.  The two most commonly used are KLH and BSA.  For most purposes, either carrier will be adequate.  At Pepceuticals we can employ other carriers of choice (i.e. ovalbumin, MSA, RSA).