Monoclonal Antibodies

Working to the sponsors specific requirements, Pepceuticals hybridoma production service will undertake the isolation and development of murine hydbridomas secreting monoclonal antibodies reactive to the antigen (synthetic or non-synthetic protein) of choice. 

Although programmes may vary, the standard procedure involving three phases will typically take 4-6 months to complete.

Procedure Day Description
Antigen Preparation Day 0 Synthesis of peptide, purification and conjugation
Control Serum Collection Day 0 Tail vein bleeds taken prior to start of immunisation (pre-immune bleed)
1st Injection Day1 Intraperitoneal injection of 10-50µg of purified peptide or protein coupled to KLH (complete Freund's adjuvant)
2nd Injection Day21 Intraperitoneal injection of 10-50µg of purified peptide or protein coupled to KLH (complete Freund's adjuvant)
3rd Injection Day 42 Intraperitoneal injection of 10-50µg of purified peptide or protein coupled to KLH (complete Freund's adjuvant)
4th Injection Day 45 Intraperitoneal injection of 10-50µg of purified peptide or protein coupled to KLH (complete Freund's adjuvant)
5th Injection Day 46 Intraperitoneal injection of 10-50µg of purified peptide or protein coupled to KLH (complete Freund's adjuvant)
Isolation of Hybridoma Day 50 Isolation by cell fusion with a non-secreting myeloma cell line
Incubation Day 50 Hybridomas will be incubated for at least two weeks followin cell fusion
ELISA1 Day 64 Verify disposition of host
ELISA2 Day 71 Re-verify disposition of host
Cryopreservation of 2 clones Day 71 Cloning of important parental cell lines, storage for 6 months and provision of 50ml of each antibody supernatant for client use