Antigen selection

AntibodiesWe are able to advise on potential antigen candidates from a peptide/ protein sequence, should this be required.  Our scientists use modern qualified methods for determining regions of a sequence that are suitable for use as an antigen candidate.

The smallest synthetic peptides that will consistently educe antibodies that bind to the original protein are 6 amino acids long.  Smaller peptides often lead to weak or no immunogenic response.  Generally peptides should be of minimum length 10 amino acids.

Antigenic response to most peptides can be induced successfully by careful synthesis, coupling and immunisation, leading to specific antibodies.  What most researchers want to know is how likely will it be that anti-peptide antibodies will recognise the native protein?

Suggestions for choosing the peptide sequences would be: If possible select more than one peptide sequence from the native protein.

  • Use the carboxy terminal sequence if it is hydrophilic and a suitable coupling group is available or can be added.
  • Use the amino terminal sequence if it is hydrophilic and a suitable coupling group is available or can be added.
  • Use internal hydrophilic regions that are perhaps longer in peptide length.